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. 2016 May 6;6:25465. doi: 10.1038/srep25465

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Effect of the method used for the blood collection: retro-orbital vessel puncture vs. tail-tip amputation. Plasma glucose levels were measured in the laboratory by the mutarotase GOD method (No. 12), and blood glucose levels were measured by each glucometer (No. 1–11). (a) Sampling in the random-fed status (N = 18). (b) Sampling conducted 15 min after oral glucose loading (N = 10). (c) Sampling conducted 30 min after intraperitoneal insulin loading (N = 8). *P < 0.05. **P < 0.01. Values are the means ± SE. Black bars, retro-orbital vessels, White bars, tail-tip.