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. 2016 May 5;7:11473. doi: 10.1038/ncomms11473

Figure 3. E3/49K is secreted and expressed on the surface of infected cells.

Figure 3

(a) Analysis of E3/49K secretion from HAdV-D28-infected cells. Molecular sizes are indicated in kDa. Full immunoblot is shown in Supplementary Fig. 8a. (b) Detection of the E3/49K protein expressed at the surface of HAdV-D28-infected cells by immunofluorescence (left panel) and flow cytometry (right histograms). Grey-filled histograms represent staining corresponding to non-infected cells, black histograms show E3/49K surface expression on HAdV-D28-infected cells at the indicated hours post infection (p.i.). Scale bar, 10 μm. (c) Binding of CD45, LILRB1 and PUNC purified ECDs, found to interact with HAdV-D28 E3/49K, to infected cells. Grey-filled histograms represent binding of a control recombinant protein, whereas black histograms show binding of the indicated E3/49K hits to the surface of infected cells. Assays shown are representative of two independent experiments.