Figure 5. The inhibitory receptor LILRB1 is a semi-conserved, functional target.

(a) Kinetic parameters calculated for the interaction between the E3 proteins and LILRB1 recombinant ECD. The χ² and R_max values indicate the goodness of the experimental fitting. (b) PBMCs were stimulated as indicated and the activation of LILRB1 was analysed in receptor immunoprecipitates using an anti-phosphotyrosine antibody. Blots were reblotted against LILRB1 to control for similar protein loading. Molecular sizes are indicated in kDa. Full immunoblot is shown in Supplementary Fig. 8f. (c) K562 target cells were exposed to primary NK cells at the indicated effector:target ratios in the absence (mock) or presence of the indicated E3 proteins (100 nM). HAdV-D53 E3/49K significantly reduced NK-mediated cytotoxicity through interference with CD45 signalling, similar to the results described for HAdV-D19 E3/49K (ref. ¹⁶). HAdV-E4 CR1β inhibited NK cell killing (*P<0.01 (analysis of variance)). Assays are representative of at least two independent assays run in triplicates. Error bars represent s.d.