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Specificity of the LAMP-qPCR device assay. All bacterial suspensions were adjusted to 106 CFU/mL and detected with real-time fluorescence loop-mediated isothermal amplification kit method using a qPCR device. (A) Out of all the strains tested, only V. alginolyticus 17749 and strain 030-002 showed amplification. (B) All the 92 strains showed “S-shaped” amplification curves. N.C as negative control, P.C as positive control.
