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Sequences of universal primers for 16s rDNA sequencing.
| Target | Primer∗ | Sequences from 5′ to 3′ |
|---|---|---|
| 16s rDNA | 27F | AGAGTTTGATCCTGGCTCAG |
| 1492R | GGTTACCTTGTTACGACTT | |
| n27F | AGAGTTTGATCMTGGCTCAG | |
| n1492R | TACGGYTACCTTGTTACGACTT | |
∗27F the forward primer and 1492R the reverse primer were for Vibrio bacteria; n27F the forward primer and n1492R the reverse primer were for non-Vibrio bacteria.
