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. 2016 May 5;7:11406. doi: 10.1038/ncomms11406

Figure 2. PD-L1 and CD80 are directly regulated by miR-424(322).

Figure 2

(a,b) PD-L1 and CD80 are potential targets of miR-424(322). The miR-424(322)-targeting sites in the 3′-UTRs of human PD-L1 and CD80 are shown. (c,d) The luciferase vectors that contain the human wild-type (WT) and mutant (MUT) PD-L1 (c) and CD80 (d) 3′-UTR regions were co-transfected into Skov3 cells with miR-424(322) or scramble miRNA precursors (miR-Scr). The relative luciferase/Renilla activities were analysed in the cells 48 h after the transfection. The results represent the mean±s.e.m. from three independent experiments. t-test, **P≤0.01. (e) Skov3 and OVA3 cells were transfected with miR-424(322) or miR-Scr. mir-424(322) mRNA levels were determined via qRT-PCR assay. t-test, **P≤0.01. The results represent the mean±s.e.m. from three independent experiments. The expression levels of PD-L1 were analysed by western blotting. One representative experiment of three experiments is shown. (f) DCs were transfected with miR-424(322) or miR-Scr. miR-424(322) mRNA levels were determined via a qRT-PCR assay. t-test, **P≤0.01. The results represent the mean±s.e.m. from three independent experiments. The expression levels of CD80 were determined by western blotting. One representative experiment of three experiments is shown.