Table 1. The intensity of immunoreactivity (GPV, TMUV, CD8α, and CD4) was scored subjectively.
| Tissue name | Brain | Lung | Liver | Small intestine | Rectum | Spleen | Heart |
|---|---|---|---|---|---|---|---|
| GPV | + | +++ | +++ | +++ | +++ | ++ | – |
| CD8α | ++ | +++ | +++ | +++ | +++ | ++ | – |
| CD4 | + | + | + | + | + | + | – |
| TMUV | +++ | ++ | +++ | +++ | +++ | ++ | ++ |
| CD8α | + | ++ | ++ | ++ | +++ | ++ | + |
| CD4 | + | + | + | + | + | + | – |
The birds were humanly killed at 5 days post infection, and the selected tissues were isolated for IHC analysis. No detectable antigen (−); weak, antigen faintly detected (+); moderate, antigen readily detected (++); strong, antigen staining intense (+++).