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. 2016 May 6;6:25550. doi: 10.1038/srep25550

Figure 5. Modification of the stalk region does not influence the biological activity of the IL-6R.

Figure 5

(a) Cell surface expression of IL-6R on stably transduced Ba/F3-gp130-IL-6R and Ba/F3-gp130-IL-6R∆ S359_S361 cells was determined via flow cytometry. (b) Equal amounts of Ba/F3-gp130-IL-6R cells were incubated for 48 h with the indicated concentration of either IL-6 or Hyper-IL-6. Cell proliferation was determined as described in Experimental Procedures. (c) Equal amounts of Ba/F3-gp130-IL-6R cells were starved for 2 h in serum free medium and stimulated with 10 ng/ml IL-6 or 10 ng/ml Hyper-IL-6 for 15 min or left untreated. Phosphorylation of STAT3 was determined by Western blotting. Total STAT3 served as endogenous loading control. (d,e) The experiments were performed as described under panels (c,d), but with stably transduced Ba/F3-gp130-IL-6R∆ S359_S361 cells. Data show one representative experiment out of three performed.