Table 2. Spontaneous revertant frequencies of unc-58(e665).
unc-58(e665) background | Trial | Plates with revertants/total plates | Mutation frequency ± SEM |
---|---|---|---|
Wild type | 1 | 0/40 | 7.06 × 10−7 ± 7.06 × 10−7 |
2 | 1/38 | ||
3 | 0/39 | ||
4 | 0/36 | ||
sws-1 | 1 | 2/41 | 2.00 × 10−6 ± 1.26 × 10−6 |
2 | 0/40 | ||
3 | 0/37 | ||
4 | 1/39 |
unc-58 reversion assay was carried out as described in Materials and Methods. A plate was scored as having a reversion event if it contained wild-type moving worms. Mutation frequency was calculated by dividing the proportion of plates with reversion events by the number of haploid genomes per plate. The data are presented as the mean mutation frequency ± SEM for four trials.