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. 2016 Feb 22;203(1):283–298. doi: 10.1534/genetics.115.186270

Figure 3.

Figure 3

T. gondii MAF1 paralog expression differs between lineages. (A and B) Polyclonal antibodies were generated specifically against the C termini of TgMAF1RHa1 (Ser173 to Ser443) or TgMAF1RHb1 (Thr159 to Asp435). Protein expression was compared by immunofluorescence across three strains representing clonotypes I, II, and III. Based on immunofluorescence and Western blotting, antibodies against TgMAF1RHa1 detected protein in all three strains, while antibodies against TgMAF1RHb1 detected protein only in RH and CTG (and not ME49). (C) Antibodies against TgMAF1RHb1 are able to detect TgMAF1RHb1 expression in transgenic type II parasites expressing the TgMAF1RHb1 protein.