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. 2016 Feb 22;203(1):283–298. doi: 10.1534/genetics.115.186270

Figure 5.

Figure 5

MAF1RHa1 and MAF1RHb1 differ in their ability to complement HMA in T. gondii and N. caninum. (A) HFFs were labeled with MitoTracker and infected with parasites transiently transfected with either HA-MAF1RHa1 or HA-MAF1RHb1. MAF1RHb1 but not MAF1RHa1 is able to confer the HMA phenotype in TgME49. (B) Identical results were obtained for N. caninum. Bar, 5.0 μm. (C) HA-MAF1RHb1 was transfected into either TgME49 (top) or N. caninum (bottom), and HA-positive clones were isolated by limiting dilution. Wild type (WT, left) and TgMAF1RHb1 complemented (right) were grown for 18 hr in HFFs and processed for electron microscopy. Asterisks indicate host mitochondria. Bar, 500 nm.