Figure 3.

The requirement for calcineurin-dependent dephosphorylation of Rod1 to promote adaptation is bypassed by nonphosphorylatable Rod1 alleles. (A) The adaptation-promoting capacity of the indicated alleles of Rod1 was assessed, as in Figure 1A, in otherwise isogenic sst2∆ tester cells that were wild type or lacked the paralogous catalytic subunits (cna1∆ cna2∆) or the small regulatory subunit (cnb1∆) of phosphoprotein phosphatase 2B/calcineurin. (B) Expression of the Rod1 variants shown in A was confirmed as in Figure 2C.