Table 1. Yeast strains used in this study.
| Strain | Genotype | Source |
|---|---|---|
| sst2∆ GEV (JT5919) | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 sst2∆::SpHIS5 leu2Δ0::GEV::NatMX | Alvaro et al. (2014) |
| snf1Δ sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 snf1Δ::KanMX4 | This study |
| kin1Δ sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 kin1Δ::KanMX4 | This study |
| kin2Δ sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 kin2Δ::KanMX4 | This study |
| kin4Δ sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 kin4Δ::KanMX4 | This study |
| kcc4Δ sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 kcc4Δ::KanMX4 | This study |
| hsl1Δ sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 hsl1Δ::KanMX4 | This study |
| frk1Δ sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 frk1Δ::KanMX4 | This study |
| gin4Δ sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 gin4Δ::KanMX4 | This study |
| cnb1Δ sst2∆ GEV (JT6694) | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 cnb1Δ::KanMX4 | Alvaro et al. (2014) |
| cna1Δ cna2Δ sst2∆ GEV (JT6695) | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 cna1Δ::KanMX4 cna1Δ::KanMX4 | Alvaro et al. (2014) |
| BJ5459 GEV (JT6743) | MATa ura3-52 trp1 lys2-801 leu2Δ1 his3∆200 pep4∆::HIS3 prb1∆1.6R can1 GAL leu2Δ1::GEV::NatMX | Alvaro et al. (2014) |
| rod1Δ rog3∆ ldb19∆ sst2∆ GEV (JT6716) | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1∆::KanMX4 rog3∆::KanMX4 ldb19∆::NatMX sst2∆::SpHIS5 | Alvaro et al. (2014) |
| STE2 sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 STE2::HPH | This study |
| STE27KR sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 STE27KR::HPH | This study |
| STE2∆296 sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 STE2∆296::HPH | This study |
| STE2-mCherry sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 | This study |
| STE27KR-mCherry sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE27KR-mCherry::URA3 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 | This study |
| STE27KR-mCherry sst2∆ GEVb | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE27KR-mCherry::ura3 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 | This study |
| STE2-mCherry bar1∆ (JT6677) | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 bar1∆::CgLEU2 | Alvaro et al. (2014) |
| STE27KR-mCherry bar1∆ | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE27KR-mCherry::URA3 bar1∆::CgLEU2 | This study |
| STE2-mCherry rod1Δ rog3∆ bar1∆ (JT6679) | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 rod1∆::KanMX4 rog3∆::KanMX4 bar1∆::CgLEU2 | Alvaro et al. (2014) |
| STE27KR-mCherry rod1Δ rog3∆ bar1∆ | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE27KR-mCherry::URA3 rod1∆::KanMX4 rog3∆::KanMX4 bar1∆::CgLEU2 | This study |
| bni1Δ sst2∆ GEVa | MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 bni1Δ::KanMX4 | This study |
a
To generate a Gal4(1-93)-estrogen receptor (ER)-VP16 chimera (designated GEV)-expressing version of the indicated yeast strain, pACT1-GEV (Veatch et al. 2009; McIsaac et al. 2011) was digested with EcoRV and introduced into the cells of interest by DNA-mediated transformation (Amberg et al. 2005), and nourseothricin-resistant colonies were selected, in which GEV (expressed under control of an ACT1 promoter) is integrated at the leu2Δ0 locus.
b
The immediately preceding strain was streaked onto plates containing 5-FOA, and a resulting Ura- (ura3) derivative was selected.