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. 2016 Feb 24;203(1):299–317. doi: 10.1534/genetics.115.186122

Table 2. Plasmids used in this study.

Plasmid Genotype Source
pEGKG GAL1prom-GST Yeast Deletion Collection (Open Biosystems, Inc.)
, URA3
pEGKG-Rod1 GAL1prom-GST Zhu et al. (2000)
, URA3
pEGKG-Rod1315Aa GAL1prom-GST This study
, URA3
pEGKG-Rod1447Aa GAL1prom-GST This study
, URA3
pEGKG-Rod1641Aa GAL1prom-GST This study
, URA3
pEGKG-Rod1706Aa GAL1prom-GST This study
, URA3
pEGKG-Rod1720Aa GAL1prom-GST This study
, URA3
pEGKG-Rod1781Aa GAL1prom-GST This study
, URA3
pEGKG-Rod1447A 641Aa GAL1prom-GST This study
, URA3
pEGKG-Rod1447A 706Aa GAL1prom-GST This study
, URA3
pEGKG-Rod13A (Rod1447A 641A 706A)a GAL1prom-GST This study
, URA3
pEGKG-Rod14A (Rod1315A 447A 641A 706A)a GAL1prom-GST This study
, URA3
pEGKG-Rod15A (Rod1315A 447A 641A 706A 720A)a GAL1prom-GST This study
, URA3
pEGKG-Rod16SA (Rod1S315A S447A S641A S706A S720A S781A)a GAL1prom-GST This study
, URA3
pGEX6P1-Rod16SE (Rod1S315E S447E S641E S706E S720E S781E)a GAL1prom-GST This study
, URA3
pGEX6P1-Rod1ARR (Rod11-402)a GAL1prom-GST This study
, URA3
pGEX6P1-Rod1TAIL (Rod1403-837)a GAL1prom-GST This study
, URA3
pGEX6P1-Rod11SA ARR (Rod11-402 S315A)a GAL1prom-GST This study
, URA3
pGEX6P1-Rod15SA TAIL (Rod1403-837 S447A S641A S706A S720A S781A)a GAL1prom-GST This study
, URA3
pEGKG-Rod12SA (Rod1138A 807A)a GAL1prom-GST This study
, URA3
pEGKG-Rod12SE (Rod1138E 807E)a GAL1prom-GST This study
, URA3
pEGKG-Rod18SA (Rod1S315A S447A S641A S706A S720A S781A 138A 807A)a GAL1prom-GST This study
, URA3
pEGKG-Rod1PANA (pJT4954) GAL1prom-GST Alvaro et al. (2014)
, URA3
pEGKG-Rod1PASA (pJT4955) GAL1prom-GST Alvaro et al. (2014)
, URA3
pEGKG-Rod1PPxY-less (pJT4956) GAL1prom-GST Alvaro et al. (2014)
, URA3
pEGKG-Rod1V/PPxY-lessa LDB19prom This study
CEN, HIS3
pEGKG-Rod12A, V/PPxY-lessa LDB19prom This study
CEN, HIS3
pEGKG-Rod16A, V/PPxY-lessa LDB19prom This study
CEN, HIS3
pEGKG-Rod18A, V/PPxY-lessa LDB19prom This study
CEN, HIS3
pEGKG-Rog3 GAL1prom-GST Zhu et al. (2000)
, URA3
pEGKG-Rog3∆400 GAL1prom-GST Alvaro et al. (2014)
, URA3
a

Generated by site-directed mutagenesis (Green and Sambrook 2012b) with synthetic oligonucleotides containing the desired codon alterations (using the wild-type sequences in pRS426 vectors as the template). DNA from the corresponding gene was amplified from genomic DNA by PCR (Green and Sambrook 2012a) and then cloned into pEGKG.