Table 1. S. cerevisiae strains.
| Strain | Genotype | Source |
|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Open Biosystems |
| yDH227 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad18ΔKANR | This study |
| yDH350 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad9ΔKANR | This study |
| yDH355 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sgs1ΔKANR | This study |
| yDH357 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2ΔKANR | This study |
| yDH359 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad50ΔKANR | This study |
| yDH452 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 exo1ΔKANR | This study |
| yDH455 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 apn1ΔKANR | This study |
| yDH456 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ctf4ΔKANR | This study |
| yDH457 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ctf8ΔKANR | This study |
| yDH460 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mph1ΔKANR | This study |
| yDH465 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1ΔKANR | This study |
| yDH492 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2T675A yku80ΔKANR | This study |
| yDH513 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 dia2ΔKANR | This study |
| yDH567 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A est3ΔKANR | This studya |
| yDH568 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2T675A est3ΔKANR | This studya |
| yDH569 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A est3ΔKANR | This studya |
| yDH576 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 srs2ΔKANR | This study |
| yDH578 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ctf4T401A, T411A | This study |
| yDH587 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad50T568A | This study |
| yDH599 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 apn1S350A, S356A | This study |
| yDH600 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 est3ΔKANR | This studya |
| yDH603 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626A ctf8ΔKANR | This study |
| yDH604 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626A rad9ΔKANR | This study |
| yDH606 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A rad27ΔKANR | This study |
| yDH607 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A pol32ΔKANR | This study |
| yDH608 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A yku80ΔKANR | This study |
| yDH610 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 pol32ΔKANR | This study |
| yDH625 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A srs2ΔNATR | This study |
| yDH627 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad27ΔKANR | This study |
| yDH628 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 clb2ΔKANR | This study |
| yDH629 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 yku80ΔKANR | This study |
| yDH638 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A | This study |
| yDH641 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A sgs1ΔKANR | This study |
| yDH650 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad18T155A, T282, S284A | This study |
| yDH654 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A ctf4ΔKANR | This study |
| yDH664 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mph1T540A, S542A, S543A | This study |
| yDH672 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626A | This study |
| yDH685 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A,T675A apn1ΔKANR | This study |
| yDH687 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rtt109ΔKANR | This study |
| yDH690 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A rtt109ΔKANR | This study |
| yDH693 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626A srs2ΔKANR | This study |
| yDH698 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A clb2ΔKANR | This study |
| yDH700 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626A dia2ΔKANR | This study |
| yDH702 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A rad17ΔKANR | This study |
| yDH706 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A rad24ΔKANR | This study |
| yDH714 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A exo1ΔKANR | This study |
| yDH730 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sae2ΔKANR | This study |
| yDH741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A mec1ΔLEU2 sml1ΔHIS3 sae2ΔKANR | This study |
| yDH751 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sae2ΔHIS3 | This study |
| yDH752 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A sae2ΔHIS3 | This study |
| yDH753 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2T675A sae2ΔHIS3 | This study |
| yDH754 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A T675A sae2ΔHIS3 | This study |
| yDH755 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2ΔURA3 sae2ΔHIS3 | This study |
| yDH794 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A yku80ΔKANR | This study |
| yDH805 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A T675A tel1ΔKANR sae2ΔHIS3 | This study |
| yDH806 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1ΔURA3 sml1ΔHIS3 rad9ΔKANR | This study |
| yDH807 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1ΔURA3 dia2ΔKANR | This study |
a
EST3 gene was deleted by PCR. The freshly made est3Δ transformants were grown in YPD for 14 hr (approximately eight generations) before being used for experiments and being frozen and stored at −80°C.