Table 3. Genetic interactions of tof1S379A, S626A and xrs2S349A, T675A.
| Genetic interactions testeda | Functions | Genetic interactions observed |
|---|---|---|
| tof1S379A, S626A | ||
| ctf8Δ | Required for sister-chromatid cohesion | No |
| srs2Δ | DNA helicase; involved in HR and sister-chromatid cohesion | No |
| rad9Δ | DNA damage-dependent checkpoint | Yes |
| dia2Δ | F-box protein; required for deactivation of Rad53 checkpoint kinase | Yes (suppression) |
| xrs2S349A, T675A | ||
| sgs1Δ | RecQ family DNA helicase; involved in HR | No |
| exo1Δ | 5′-3′ exonuclease; involved in HR | Yes |
| rtt109Δ | Histone acetyltransferase; involved in NHEJ | No |
| yku80Δ | Ku complex; involved in NHEJ and telomere function | Yesb |
| clb2Δ | B-type cyclin involved in cell cycle progression | No |
| pol32Δ | Third subunit of DNA polymerase delta | No |
| rad27Δ | Flap endonuclease; required for DNA replication and BER | No |
| apn1Δ | Apurinic/apyrimidinic endonuclease; required for BER | No |
| ctf4Δ | Required for sister-chromatid cohesion; DNA replication | No |
| srs2Δ | DNA helicase; involved in HR and sister-chromatid cohesion | No |
a
The genetic interactions with tof1S379A, S626A and xrs2S349A, T675A were selected from the manually curated genetic interactions with tof1Δ and xrs2Δ in the Saccharomyces Genome Database (http://www.yeastgenome.org/).
b
xrs2S349A, T675A yku80Δ showed enhanced growth defects (but not MMS sensitivity) as compared to yku80Δ.