Figure 1.

angur is an allele of σ₂-adaptin, the smallest subunit of adapter protein 2 (AP2) complex. (A) Deficiency mapping showed the angur locus to be located in the region of 93D₁–93D₄ on the third chromosome of Drosophila. The three deficiency lines that uncovered the angur locus—Df(3R)BSC677, Df(3R)ED10838, and Df(3R) ED10845—are labeled. Solid bars mark the deleted regions in these deficiency lines. The 93D₁–93D₄ region of the chromosome contains at least 10 identified genes. Different colored arrows represent the relative positions and orientations of the genes in the 93D₁–93D₄ region. While angur⁷ and angur² were obtained from mobilization of EP0877, angur⁵ was obtained from mobilization of KG06118b. The P-element in angur alleles was located in the 5′ end of the hsrω gene but did not affect expression of the hsrω gene. (B) Genomic organization of the σ₂-adaptin locus showing exons (represented by solid boxes) and introns (represented by thin lines). The transcription start site is represented by an arrow, and the untranslated regions are shown by black solid boxes. The insertion site of P-element KG02457 lies in the third exon of the gene. (C) Semiquantitative (top panel) and quantitative RT-PCR (bottom panel) depicting transcript levels of σ₂-adaptin in controls, angur⁷/AP2σ^KG02457, angur⁷/angur², angur⁷/angur⁵, actin5C-Gal4-driven σ₂-adaptin RNAi, and rescued heteroallelic mutant (actin5C/+; angur⁷/UAS-AP2σ, AP2σ^KG02457), respectively. σ₂-adaptin transcript level is dramatically reduced in the heteroallelic mutants or actin5C-Gal4-driven σ₂-adaptin RNAi. rp49 transcript level was used as an internal concentration control for messenger RNA (mRNA). The error bars in the bottom panel represent SEM. Statistical analysis based on one-way ANOVA followed by post-hoc Tukey’s multiple comparison test. (D) Rescue of AP2σ^KG02457 mutants and heteroallelic combinations in homozygous or transheterozygous combinations using ubiquitous actin5C-Gal4 or the neuronal elav^C155-Gal4 drivers.