Figure 2.

Functions of ebony, black, tan, and yellow in different body regions of Oncopeltus adults. (A1–A5) Wild-type Oncopeltus shows alternating black-orange patterning. Black pigmentation is present in the head (A2), thorax (A3), ventral abdomen (A4), and dorsal abdomen (A5). (B1–B5) The ebony RNAi adult phenotypes showed the expansion of black pigment in the head (B2) and thorax (B3), whereas such an expansion was only moderate in the ventral abdomen (B4) and barely noticeable in dorsal abdomen (B5). (C1–C5) The black RNAi adults showed a phenotype similar to the ebony RNAi individuals described earlier. (D1–D5) The tan RNAi adult phenotypes showed a differential defect of black pigment across the body. The defect of black was subtle in the head (D2) and thorax (D3), while it was quite significant in ventral abdomen (D4). Also, on the dorsal A1 segment, the two black patches were reduced (arrows, D5). (E1–E5) The yellow RNAi adults showed varying levels of reduction in black pigment between body regions. Reduction was moderate in the head (E2) and thorax (E3) whereas quite significant in ventral abdomen (E4). The two black patches on the dorsal A1 segment were also significantly reduced (arrows, E5). Scale bars, 1 mm (A1, B1, C1, D1, and E1) and 500 µm (A2–A5, B2–B5, C2–C5, D2–D5, and E2–E5).