Functions of ebony, black, aaNAT, yellow, and tan in the forewing and hindwing of Oncopeltus adults. (A1–A2) Pigmentation patterns of wild-type wings. (B1–B2) The ebony RNAi adults showed an expansion of black pigmentation into the orange subregion of the forwing (B1), while the hindwing remained unaffected (B2). (C1–C2) The black RNAi adult wings showed a similar phenotype to the ebony RNAi adults, as described earlier. (D1–D2) The aaNAT RNAi adult phenotypes show no effect on the forewing (D1), while melanization expanded into the anal region of the hindwing (asterisk in D2). (E1–E2) The yellow RNAi adult wing phenotype showed a reduction of black pigment on the forewing (E1) and the hindwing (E2). (F1–F2) The tan RNAi adult phenotype showed no noticeable pigmentation effect in the forewing and hindwing. FW, forewing; HW, hindwing. Scale bars, 1 mm.