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Proliferation of MSCs with the two protocols. (A) Rate of population doubling during expansion from passage 2 to passage 5. (B) Cell numbers of passage 5 Protocol A and B cells expanded for 96 h in growth medium. (C) Cell numbers in Protocol A and B samples in growth, osteogenic and adipogenic media after 14 days, and in chondrogenic pellet cultures after 21 days. (D) Percentage of senescent cells in Protocol A and B populations; *p < 0.05
