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. 2016 Jan 11;9(4):414–424. doi: 10.1002/jbio.201500258

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© 2016 The Authors. Journal of Biophotonics published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Region of interest selection for data analysis. Rendered 3‐D images of 24 hpf Tg(Ubi : Caspase3bios) zebrafish following 25 Gy gamma irradiation, derived from FBP reconstruction and pixelwise fitting in FLIMfit to a single exponential decay model of (A) whole FLIM OPT data set, showing intensity merged false colour lifetime (B) whole FLIM OPT data set, showing false colour lifetime, without intensity merging, to highlight short lifetime contribution of yolk and (C) FLIM OPT data set showing the 2‐D slices through the ROI of the reconstructed fluorescence lifetime image of the segmented dataset.