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. 2016 Jan 11;9(4):414–424. doi: 10.1002/jbio.201500258

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© 2016 The Authors. Journal of Biophotonics published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Irradiation causes an increase in fluorescence lifetime at 3.5 hours in the head region of zebrafish. 24 hpf Tg(Ubi : Caspase3bios) were irradiated (C–F) with 25 Gy from a ¹³⁷Cs source, or left untreated (A–C) and imaged at 1 hpi (A, D), 2 hpi (B, E) and 3.5 hpi (C, F) with FLIM OPT. 5 time gated images were acquired every 4°, through a full 360° rotation. Data was reconstructed using MATLAB, and fitted using a single pixelwise variable projection model in FLIMfit, giving the mean lifetime per pixel. 3D images were generated using the 3D viewer plug‐in in FiJi. Histograms show frequency of pixel lifetimes. Centre histograms: head region. Edge histograms: tail region.