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. 2016 May 6;17:94. doi: 10.1186/s13059-016-0952-x

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© Pandolfini et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a ES cell in vitro neuralization and RNA analysis. DIV days of in vitro differentiation. b The fraction of total mRNAs that are up- or down-regulated between consecutive steps of differentiation, with a threshold of |log₂FC| > 2.5 (where FC is fold change). c Number of genes whose mRNA is significantly released (or loaded) by Argonaute (Ago; see “Methods”); ***p = 0.001 (χ²-test). IP immunoprecipitation