Fig. 4.

a Upper panel: the distribution of Ago enrichment variation (ΔE) during the ES–ELA transition. The most negative side of the x-axis (ΔE < −5), containing few values (density < −0.002), is not shown for clarity (for comparison, see Additional file 1: Figure S1e). Middle and lower panels: comparison between log₂ variation of ribosome enrichment (RiboΔE, middle panel) or log₁₀ protein fold changes (lower panel) of three subsets of genes displaying significant Ago release (ΔE ≤ −2, green), significant Ago loading (ΔE ≥ 2, pink) or non-significant change of Ago enrichment (−1 ≤ ΔE ≤ 1, gray). Asterisks indicate the p value (Student’s t-test, ***p = 0.001) against the null hypothesis of mean equal to 0. b–d Total mRNA fold change (Tot mRNA), linear Ago enrichment (Ago En), ribosome enrichment (Ribo En), and MS fold change (Protein) for the members of DNMT, KDM, and SWI/SNF classes of chromatin regulators detected in ES and ELA nuclear cell extracts. The plus sign marks values that are slightly out of the ranges indicated in “Methods” but are still relevant. Other genes unrelated to these families but displaying similar regulation are listed in Additional file 4: Table S3. **p = 0.01, ***p = 0.001 (Wilcoxon test). Error bars show standard error