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. 2016 May 5;9:101. doi: 10.1186/s13068-016-0516-z

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© Wu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Overexpression of a PvSPL2 chimeric repressor in switchgrass. a Morphological characterization of PvSPL2SRDX-overexpressing transgenic switchgrass plants. Representative plants from group I (TPvSPL2SRDX1) and group II (TPvSPL2SRDX4) are shown. Control plant was produced with pANIC6B empty vector from the same batch of experiment. b Expression levels of PvSPL2 in transgenic switchgrass plants were revealed by qRT-PCR. Switchgrass Ubq2 was used as the reference for normalization. PvSPL2: sum of exo- and endo-PvSPL2 transcript versions. c Tiller number of transgenic switchgrass plants. d Dry matter biomass yield of transgenic switchgrass plants. The transgenic and control plants were harvested after 4-month growth in the greenhouse. Values are mean ± SE (n = 3)