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. 2016 May 5;17:205. doi: 10.1186/s12891-016-1055-2

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© Suzuki et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Cytokine mRNA expression in bone marrow derived-macrophages. Bone marrow derived-macrophages were obtained from the bone marrow cells of wild-type (n = 19) and Padi4^−/− CIA mice (n = 17) 10 days after booster injection. The cells were then analyzed by real-time TaqMan RT-PCR for mRNA levels of (a) tumor necrosis factor alpha (TNF-α), b interleukin (IL)-1β, c Granulocyte-macrophage colony-stimulating factor (GM-CSF), and d IL-6. Relative units were obtained from the comparative ΔΔC_T analysis. * P < 0.05, **P < 0.01 (Student’s t-test)