Fig. 7.

Immunohistochemical localization of citrullinated fibrinogen in synovial tissues of collagen-induced arthritis (CIA) mice. Immunohistochemistry using anti-citrullinated fibrinogen antibody (ModiQuest) and DAB staining of the lining and sublining in an inflamed synovial tissue from wild-type CIA mice (n = 3, the scores are 4, c–e) and Padi4^−/− CIA mice (n = 3, the scores are 4, h–j) and in a synovial tissue from wild-type control mice (n = 1, a) and Padi4^−/− control mice (n = 1, f). Staining of a matched irrelevant rabbit IgG-negative control performed at the same concentration (b and g). (a–c, f–h) Magnification, ×40. (d, e, i, j) Magnification, ×100