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Profiling arginine methylation of quaking protein. (A) MS/MS spectrum of mono-methylated R242 residue identified from SK-Hep1+ cells using the Me-R4-100 antibody. Y15 ion corresponding to the methylated-R residue is highlighted. (B) The activation of p27kip1, a target of QKI, was assessed by Western blotting suggesting that methylation might increase QKI-5 activity as no change on its steady-state levels were detected.
