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Sequence and purification of peptide concatenated standards. (A) Sequence of PCS 1 and PCS 2. White: standard peptides. Gray: flanking residues added to provide natural context for trypsin cleavage. Color-coding for the individual needle complex components as indicated in the legend. (B) SDS-PAGE of MBP-PCS1 and MBP-PCS 2 with upper 10% acrylamide gel for separation and lower 4% acrylamide gel for extraction.
