Heatmap from vaccine trial. In 2009, six volunteers received the seasonal trivalent influenza vaccine (A/Brisbane/59/2007, A/Brisbane/10/2007, B/Brisbane/60/2008) donated blood before and 21 days following receipt of the vaccine. 28 peptides were selected using a paired t test to find peptides different on day 21 versus prevaccine (p < 4.60 × 10−16). These 28 peptides were filtered to find 21 that were higher on day 21 for every volunteer (see Supplemental Table S5). For patient 73, we collected only two time points, and for patients 112 and 113, we were unable to draw blood on day 2. Each patient has slightly different peptides that were most reactive against the influenza vaccine components. Graph to the right is the sum of all fluorescence intensity. Error bars represent the upper and lower 95% CI.