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. 2016 Mar 4;15(5):1710–1727. doi: 10.1074/mcp.M116.058131

Fig. 6.

Fig. 6.

Although Hsp70·ATP protects Tomm34 peptides covering TPR1 domain, Tomm34 binding to Hsp70·ATP induces structural changes at the αA/αB helices in Hsp70 SBD. A, difference in deuteration of Tomm34 in the complex with Hsp70 and Tomm34 alone in the presence (red) or absence (blue) of ATP after 10 min (left) or 3 h (right) of incubation in deuterated buffer. B, deuteration kinetics of selected Tomm34 peptides protected by Hsp70 in the presence of ATP, but not in nucleotide-free state. C, difference in deuteration of Hsp70 in the complex with Tomm34 and Hsp70 alone in ATP-bound (red) or nucleotide-free state (blue) after 10 min or 3 h of incubation in deuterated buffer. Hsp70 peptides 519–529 and 533–543 exhibited consistent protection by Tomm34 in the presence of ATP. D, deuteration kinetics of 519–529/533–543 peptides of Hsp70·ATP protected from deuteration by the presence of Tomm34. E, peptides are indicated in red in the tertiary structure representation of DnaK·ATP (PDB code 4jne) with human Hsp70 numbering. Hsp70 domain constitution is colored similarly as in Fig. 4.