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Peptides covering TPR1 and TPR2B domain of Hop are consistently protected by Hsp70. A, difference in deuteration of Hop in the complex with Hsp70 and Hop alone after 30 min of incubation in deuterated buffer. B, deuteration kinetics of selected Hop peptides shows stable protection by Hsp70. C, effect of Hop binding on deuteration of Hsp70 peptides after 30 min of incubation in deuterated buffer.
