Fig. 9.

Interdomain linker of Tomm34 represents important region for conformation-specific Hsp70/Tomm34 interaction. The length of TPR domain constructs and mutated residues in the linker region are indicated. Purified SBP-Hsp70 WT was preincubated with streptavidin-agarose beads and mixed with TPR constructs (A) or Tomm34 mutants (B) in different nucleotide conditions. After washing, eluted proteins were subjected to SDS-PAGE and Western blot with mouse polyclonal sera against Tomm34 (A) or silver staining (B). The capacity of polyclonal sera to detect purified TPR domains was tested in A.