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. 2016 Feb 5;291(19):10067–10077. doi: 10.1074/jbc.M115.708354

FIGURE 3.

FIGURE 3.

HCV NS5B initiation with GTP versus pGG dimer in the presence of NNIs. The experimental setup for RNA initiation with either GTP or pGG dimer is depicted in Scheme 1. The time course of primer extension and elongation complex formation (10-mer for GTP start and 9-mer for pGG start) was monitored on PAGE gel. The arrow denotes the abortive intermediates (5-mer), which appear in the presence of GS-9669 in both assays. In the presence of GS-026916, GS-46029, and GS-340494, elongation complex formation was slightly inhibited in assays started with the pGG dimer, but no significant product formation was seen in assays started with GTP.