FIGURE 7.

Stability of the elongation complex in the presence of NNI2. A, a PAGE gel displaying the stability of the NNI2 bound elongation complex in the elongation buffer with and without heparin. The elongation complex preformed in the presence of NNI2 was incubated in the elongation buffer with and without 0.2 mg/ml heparin. The remaining activity of the elongation complex was examined by reacting with 50 μm CTP for 20 s at indicated incubation time intervals. B, the fraction of the 10-mer primer extension product formation was plotted versus incubation time.