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. 2016 Mar 8;291(19):10332–10346. doi: 10.1074/jbc.M116.717538

FIGURE 7.

FIGURE 7.

Increased cellular Hsp90 activity accelerates HIV transcription in persistently infected cells. A, nitric-oxide synthase (NOS) activity in 8E5/LAV cells at 37 and 39.5 °C with thapsigargin (Thaps.) as a positive control at 37 °C. Data represent mean ± S.E. (n = 3). B, quantitative fluorescence Western blotting analysis of 8E5/LAV cell lysates. Results shown are representative of four independent experiments. C, antiviral activity of 17-AAG in 8E5/LAV cells at 37 and 39.5 °C. Mean cytotoxicity at 37 °C (5.2 μm) and 39.5 °C (5.2 μm) is shown. D, antiviral activity of 17-AAG on 8E5/LAV cells pretreated with IUdR at 37 and 39.5 °C. Mean cytotoxicity at 37 °C (5.6 μm) and 39.5 °C (5.3 μm) is shown. E, cell-associated HIV RNA from 8E5/LAV cells pretreated with IUdR at 37 and 39.5 °C. p = 0.26 at 100 nm 17-AAG and p = 0.34 at 32 nm 17-AAG by Student's t test. Data represent mean ± S.E. (error bars) (n = 3).