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. 2016 Mar 18;291(19):10363–10371. doi: 10.1074/jbc.M116.717694

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Effect of kindlin-3 knockdown on integrin conformation. A, influence of kindlin-3 expression on integrin α4β1 conformation. FRET between β1 I domain and the plasma membrane was analyzed. The FRET efficiency of K562-α4β1 control cells and transfectants in 1 mm Ca²⁺/Mg²⁺ or 1 mm Mn²⁺ was determined. B, influence of kindlin-3 expression on α4β7 conformation. FRET between β7 I domain and the plasma membrane was analyzed. The FRET efficiency of K562-α4β7 control cells and transfectants in 1 mm Ca²⁺/Mg²⁺ or 1 mm Mn²⁺ was determined. C, influence of kindlin-3 expression on CD45 conformation. FRET between CD45 and the plasma membrane was analyzed. The FRET efficiency of K562-α4β1 control cells and transfectants in 1 mm Ca²⁺/Mg²⁺ or 1 mm Mn²⁺ was determined. Error bars represent ±S.D. (n = 10). ***, p < 0.001; NS, not significant (two-tailed Student's t test).