FIGURE 6.

Phenotypes associated with rcy1Δ mutant. A, increased chromosome loss in rcy1Δ mutant cells. Sectoring assay was done as described (17). Actively growing wild-type or rcy1Δ mutant cells were plated onto YPD plates and incubated for 48 h at 30 °C. Quantification of three independent experiments is shown in the upper panel (p value 0.023), and representative images from the sectoring assay are shown in lower panels. B, rcy1Δ mutant is cold-sensitive. Mid-log phase yeast cultures were spotted onto the plates in serial 10-fold dilution and incubated at either normal temperature, 30 °C, or 16 °C as indicated. The genotypes of the strains are shown on the left of the panels.