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. 2016 Mar 9;291(19):10426–10436. doi: 10.1074/jbc.M115.697789

TABLE 2.

Binding constant of CCA+ and CCA− tRNAs to cytochrome c

Human tRNAMet with (CCA+) or without (CCA−) the CCA sequence, and labeled with Cy3 at the 5′-end, were tested for the binding with bovine and yeast cytochrome c (cyt c). Data shown are mean ± S.D.

tRNA CCA end Label Kdm)
Bovine cyt c Yeast cyt c
htRNAMet CCA+ 5′-Cy3 4.8 ± 0.7 4.8 ± 0.9
htRNAMet CCA− 5′-Cy3 3.7 ± 0.4 7.0 ± 2.0