Table 1. mRNA expression in cornea.
| MMP-9 | HO-1 | PGS-2 | IL-6 | TNF-α | |
|---|---|---|---|---|---|
| NT |
11.3±1.9 |
10.5±1 |
9.6±0.6 |
18.6±3.8 |
9.1±0.7 |
| Vehicle |
8.±1.7 |
9.8±0.7 |
9.1±0.8 |
15.7±2.6 |
9.6±0.7 |
| 2-OHE2 | 9.4±0.4 | 9.8±0.7 | 8.9±0.7 | 16.4±2.2 | 9.2±0.7 |
The relative expression level of mRNAs was determined by real-time RT–PCR and was estimated using the ΔCt value. ΔCt value was calculated using Ct (cycle threshold) values in each gene and GAPDH (ΔCt value=Ct value of each gene - Ct value of GAPDH). If the ΔCt value is lower, the relative RNA level is higher. Results are expressed as the mean ± SD (n=6).