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. 2016 Apr 20;5(4):e002865. doi: 10.1161/JAHA.115.002865

Figure 2.

Figure 2

TREK‐1 is highly expressed in mouse SAN. A, Immunoblots and (B) densitometric measurments (normalized to GAPDH loading control) showing expression of TREK‐1, Cx43, and HCN4 in detergent‐soluble lysates from mouse LV, LA, and SAN (LA and SAN lysates are from 3 pooled samples from 3 preparations, thus no error bars are provided for densitometric measurements). TREK‐1 immunoblot was performed using C‐terminal antibody. C, Confocal images of mouse RA tissue section (14 μm thick) immunostained for Cx43 and HCN4 to show intact SAN region (HCN4‐positive, Cx43‐negative area). Scale bar=100 μm. Higher magnification images (scale bar=50 μm) show sections from same region immunostained for HCN4 and TREK‐1. D, Immunoblots and (E) densitometric measurments (normalized to GAPDH) showing expression of TREK‐1 and Cx43 in detergent‐soluble lysates from rabbit LV and RV, LA and RA, and SAN (LA, RA, and SAN are from 3 pooled samples from 3 preparations). Cx43 indicates connexin 43; LA, left atrium; LV, left ventricle; RA, right atrium; RV, right ventricle; SAN, sinoatrial node.