Skip to main content
. 2016 Apr 5;5(4):e003336. doi: 10.1161/JAHA.116.003336

Figure 4.

Figure 4

Impact of Rnd3 overexpression or depletion on RhoA, Rac1, and Cdc42 activation by thrombin. A, RhoA‐GTP activation in mock‐transfected or MAT‐FLAG‐Rnd3‐transfected HUVEC monolayers during baseline conditions and 5, 30, 60, and 120 minutes following the addition of 1 U/mL thrombin. B and C, Rac1 and Cdc42 activation, respectively, under the same conditions. D, RhoA activation in HUVEC treated with Rnd3 siRNA or siCONTROL nontargeting RNA under baseline conditions and after treatment with 1 U/mL thrombin for 5, 30, 60, or 120 minutes. E and F, Activation of Rac1 and Cdc42 under the same conditions. *P<0.05 vs baseline. † P<0.05 vs. MAT‐FLAG‐Rnd3, same time point. § P<0.05 between groups, same time point. The numbers in parentheses at each time point indicate the number of replicates for the mock and MAT‐FLAG‐Rnd3 groups, respectively, in (A through C) and for the siCONTROL and Rnd3 siRNA groups, respectively, in (D through F). HUVEC indicates human umbilical vein endothelial cells.