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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Neurooncol. 2015 Sep 16;126(1):27–36. doi: 10.1007/s11060-015-1936-5

Fig. 2.

Fig. 2

Test the ability of four mutant-specific primers and two reverse primers for amplification of K27M mutant and wild type H3F3A. (a) Effects of four mutant-specific primers and two reverse primers on the amplification of wild type and K27M mutation-containing H3F3A. PCR reactions were set up using either Reverse Primer 1 (Rev 1) or Reverse Primer 2 (Rev 2). For subsequent experiments, Rev 1, which gave rise to the large ΔCT in amplification of wild type H3F3A (WT) and H3F3A K27M mutant (MT) allele, was used. (b-c) Amplification selectivity using two different amounts of H3F3A cDNA templates 1 ng (b) and 0.1 ng (c). Calculated TM of each mutant-specific primer is indicated on the X-axis.