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Effects of blocker oligo concentrations on the amplification efficiency of wild type and H3F3A K27M mutant allele. (a-b) Amplification selectivity increases with elevated blocker oligo concentrations. Blocker concentration ranging from 1X to 4X of the PCR primer concentration (a) and from 3X to 6X of the PCR primer concentration (b) was tested. (c) Amplification selectivity at a lower concentration of templates. Concentrations of blocker oligo are indicated in the figure.
