Fig. 5.

Beta1-integrin is degraded and locates to the perinuclear region.

All treatments were performed with 4.3 μM LecB. (A) Cells were treated as indicated for different time periods and β1-integrin protein levels were determined by Western blot analysis and densitometric quantification using ImageJ. Protein levels were normalized to actin. Representative blots (above) and quantification data (below) are depicted. Values represent the mean of at least three independent experiments ± SEM. Asterisks indicate the statistical significance. (B) Cells were treated as indicated for 1 h and 5 h and analyzed by confocal fluorescence microscopy with immunostainings for β-catenin (green), β1-integrin (red) and counterstaining for DNA (DAPI, blue).