Figure 1.

Increased cardiac mitochondrial H₂O₂ production in high‐fat high‐sucrose (HFHS) diet–fed mice is prevented by transgenic expression of mitochondrial catalase (mCAT). The mitochondrial H₂O₂ production rate is increased in cardiac mitochondria from wild‐type (WT) but not mCAT mice. A, H₂O₂ production rate with a complex I substrate (5 mmol/L pyruvate+5 mmol/L malate); (B) H₂O₂ production rate with a complex II substrate (5 mmol/L succinate) and inhibition of reverse electron transport (2 μmol/L rotenone). Values are mean±SEM; n=4 to 5; **P<0.01 vs WT control diet (CD); ***P<0.001 vs WT CD; ^## P<0.01 vs WT HFHS; ^### P<0.001 vs WT HFHS.