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. 2016 Jan 11;5(1):e002555. doi: 10.1161/JAHA.115.002555

Figure 7.

Figure 7

Oxidation of succinate dehydrogenase B (SDHB) Cys100 and Cys103 inhibits mitochondrial complex II activity. Exposure of cells to high‐glucose/high‐palmitate (HGHP) media (14 hours) decreased complex II activity, measured as per Figure 4, in HEK 293T cells. SDHB in which Cys100Ser (C100S), Cys103Ser (C103S), or Cys115Ser (C115S) was mutated to a redox‐insensitive serine was expressed in HEK 293T cells, and complex II activity was measured after exposure to HGHP media. A, HGHP treatment decreased complex II activity in HEK cells transfected with empty vector (CTRL), and activity was restored after incubation of cell lysate with dithiothreitol (DTT; 5 mmol/L). B, Expression of the SDHB C100S mutant prevents the HGHP‐mediated decrease in complex II activity. C, Expression of the SDHB C103S mutant prevents the HGHP‐mediated decrease in complex II activity. D, Expression of the SDHB C115S mutant has no effect on the HGHP‐mediated decrease in complex II activity. Values are mean±SEM; n=3 to 4; *P<0.05 vs CTRL; # P<0.05 vs CTRL+HGHP; ## P<0.01 vs CTRL+HGHP.