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. 2016 Jan 8;5(1):e002610. doi: 10.1161/JAHA.115.002610

Figure 4.

Figure 4

Interferon (IFN)β interferes with the recruitment of inflammatory peripheral immune cells into the ischemic brain. C57BL/6 mice (n=7/group) subjected to middle cerebral artery occlusion/reperfusion (MCAO/R) were treated with vehicle or IFNβ 3 hours after reperfusion. Forty‐eight hours later, ischemic brains of vehicle and IFNβ‐treated MCAO/R mice and sham control brains were harvested. The contralateral and ipsilateral hemispheres of vehicle‐ and IFNβ‐treated mice as well as sham control brains were subjected to mononuclear cell isolation by 30%:70% Percoll gradient. Cells were then stained with antibodies against CD45, CD11b, and Ly6G. A, top, central nervous system–infiltrating immune cells (CD45hi CD11b+) and MG (CD45int CD11b+). A, bottom, neutrophils (CD11b+Ly6G+). B and C, Numbers and percentages of peripheral infiltrating cells (CD45hi CD11b+) and neutrophils (CD11b+Ly6G+) were determined. ***P<0.001 by 1‐way ANOVA with Bonferroni's multiple comparison test.