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. Author manuscript; available in PMC: 2016 May 6.
Published in final edited form as: Cytometry A. 2015 Apr 30;87(10):897–907. doi: 10.1002/cyto.a.22683

Figure 2.

Figure 2

Recovered BMVEC from BBB Model are viable and express JAM-A. BMVEC were collected from the BBB model following treatment with Trypsin, TrypLE, heating at 55 °C for 10 min, or by vigorous scraping in three individual experiments. (A) Cell viability was assessed with Annexin V and 7AAD. Representative histograms demonstrate the fluorescent signal for each of the indicated stains. A gate was made on the positive peak to enumerate the frequency of nonviable cells. (B) Representative histograms demonstrate the PE signal for JAM-A (open peak), its isotype-matched negative control (filled peak), and their respective mean fluorescence intensities (MFI).