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. Author manuscript; available in PMC: 2016 May 6.
Published in final edited form as: Cytometry A. 2015 Apr 30;87(10):897–907. doi: 10.1002/cyto.a.22683

Figure 4.

Figure 4

BMVEC from BBB model are responsive to stimuli. BMVEC were recovered from the BBB using TrypLE following 24-h treatment with 10 ng/mL LPS or VEGF, or PBS vehicle control. Histograms from one representative experiment demonstrate the (A) PE signal for ICAM, (C) APC signal for occludin, or the appropriate isotype-matched control, and their respective MFI. After subtracting the contribution of the isotype control, the pooled fold change in (B) ICAM MFI following LPS treatment or (D) occludin MFI following VEGF treatment (filled bar) relative to vehicle (open bar, set to 1) from five or three independent experiments, respectively, was determined. Data are represented as mean ±standard error of the mean. Significance was determined by a two-tailed paired t-test. *P <0.05. **P <0.01.